Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisae
Artikel i vetenskaplig tidskrift, 2010

Saccharomyces cerevisiae has in several cases been proven to be a suitable host for the production of natural products and was recently exploited for the production of non-ribosomal peptides. Synthesis of non-ribosomal peptides (NRPs) is mediated by NRP synthetases (NRPSs), modular enzymes, which are often organized in enzyme complexes. In these complexes, partner NRPSs interact via communication-mediating domains (COM domains). In order to test whether functional interaction between separate NRPS modules is possible in yeast we constructed a yeast strain expressing two modules with compatible COM domains from two plasmids. Successful production as well as secretion of the expected dipeptide was detected. This opens the possibility of using yeast as a eukaryotic platform for fast assessment of new module combinations for the development of novel NRP compounds.

secondary metabolism

non-ribosomal peptide synthetase

COM domain

yeast

Författare

Verena Siewers

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Rita San-Bento

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Biotechnology and Bioengineering

0006-3592 (ISSN) 1097-0290 (eISSN)

Vol. 106 841-844

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Bioinformatik och systembiologi

Annan industriell bioteknik

DOI

10.1002/bit.22739