ChIP-exo and CRISPRi/a illuminate the role of Pdr1 and Yap1 in acetic acid tolerance in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2025

Budding yeast Saccharomyces cerevisiae has great potential as a host organism for various biorefinery applications. Nevertheless, the utilization of renewable plant biomass as feedstock for yeast in industrial applications remains a bottleneck, largely due to the presence of inhibitory substances such as acetic acid that are released in the biomass pretreatment processes. Exposure to acetic acid leads to different cellular stress mechanisms, several of which are directed by transcription factors. In this work, the role of the transcription factors Pdr1 and Yap1 in acetic acid tolerance was investigated using ChIP-exo and CRISPR interference/activation (CRISPRi/a). Pdr1 is the main regulator of the pleiotropic drug response, whereas Yap1 governs the oxidative stress response. CRISPRa targeting YAP1 for overexpression conferred a higher specific growth rate S. cerevisiae, whereas CRISPRi-based downregulation of PDR1 proved to be beneficial for growth in medium containing acetic acid. ChIP-exo experiments showed increased binding of Pdr1 or Yap1 to their target promoters in the presence of acetic acid, and a large number of promoters were bound by either transcription factor. Promoters genes involved in amino acid synthesis or encoding ABC transporters had the highest level of binding enrichment in the presence of acetic acid. The results highlight the potential for developing more acetic acid-tolerant yeast by altering the expression transcription factor-encoding genes and demonstrate how expression can be fine-tuned by CRISPRi/a.

stress responses

yeast

transcription factor

inhibitor stress

expression

Författare

Ibai Lenitz Etxaburu

Chalmers, Life sciences, Industriell bioteknik

Christoph Sebastian Börlin

Chalmers, Life sciences, Systembiologi

Luca Torello Pianale

Chalmers, Life sciences, Industriell bioteknik

Darshan Balachandran

Student vid Chalmers

Jens B Nielsen

Chalmers, Life sciences, Systembiologi

Florian David

Chalmers, Life sciences, Systembiologi

Verena Siewers

Chalmers, Life sciences, Systembiologi

Yvonne Nygård

Chalmers, Life sciences, Industriell bioteknik

Applied and Environmental Microbiology

0099-2240 (ISSN) 1098-5336 (eISSN)

Vol. In Press

Kartläggning av stressreglering i jäst genom CRISPRi

Vetenskapsrådet (VR) (2018-04713), 2019-09-01 -- 2023-08-31.

Ämneskategorier (SSIF 2025)

Molekylärbiologi

DOI

10.1128/aem.01824-24

PubMed

40035556

Mer information

Senast uppdaterat

2025-03-17