FadR-Based Biosensor-Assisted Screening for Genes Enhancing Fatty Acyl-CoA Pools in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2019

Fatty acid-derived compounds have a range of industrial applications, from chemical building blocks to biofuels. Due to the highly dynamic nature of fatty acid metabolism, it is difficult to identify genes modulating fatty acyl-CoA levels using a rational approach. Metabolite biosensors can be used to screen genes from large-scale libraries in vivo in a high throughput manner. Here, a fatty acyl-CoA sensor based on the transcription factor FadR from Escherichia coli was established in Saccharomyces cerevisiae and combined with a gene overexpression library to screen for genes increasing the fatty acyl-CoA pool. Fluorescence-activated cell sorting, followed by data analysis, identified genes enhancing acyl-CoA levels. From these, overexpression of RTC3, GGA2, and LPP1 resulted in about 80% increased fatty alcohol levels. Changes in fatty acid saturation and chain length distribution could also be observed. These results indicate that the use of this acyl-CoA biosensor combined with a gene overexpression library allows for identification of gene targets improving production of fatty acids and derived products.

FadR

fatty alcohols

fatty acyl-CoA sensor

fatty acids

Författare

Yasaman Dabirian

Chalmers, Biologi och bioteknik, Systembiologi

Paulo Teixeira

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Verena Siewers

Chalmers, Biologi och bioteknik, Systembiologi

Florian David

Chalmers, Biologi och bioteknik, Systembiologi

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. 8 8 1788-1800

Ämneskategorier

Bioinformatik och systembiologi

Växtbioteknologi

Genetik

DOI

10.1021/acssynbio.9b00118

PubMed

31314504

Mer information

Senast uppdaterat

2019-09-05