Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2009

The construction of mitotically stable yeast strains for heterologous gene or pathway expression often requires chromosomal integration. However, transcription levels vary between different chromosome regions. We therefore characterized 20 different integration sites of the Sacchromyces cerevisiae genome by inserting lacZ as a reporter gene under the control of two different promoters and determining expression levels through enzyme activity measurement. An up to 8.7-fold difference was detected between the sites conferring lowest and highest expression, respectively. This opens the opportunity for modulating gene expression levels while retaining promoter and culture conditions. Copyright (C) 2009 John Wiley & Sons, Ltd.

escherichia-coli

decarboxylase

specificity

solo LTRs

precursor

Saccharomyces cerevisiae

pathway

genome

sequence

lacZ

structural gene

beta-carotene

yeast

reporter gene

Författare

D. B. Flagfeldt

L. Huang

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Yeast

0749-503X (ISSN) 1097-0061 (eISSN)

Vol. 26 10 545-551

Ämneskategorier

Industriell bioteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1002/yea.1705

Mer information

Senast uppdaterat

2018-04-20