Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae
Journal article, 2009

The construction of mitotically stable yeast strains for heterologous gene or pathway expression often requires chromosomal integration. However, transcription levels vary between different chromosome regions. We therefore characterized 20 different integration sites of the Sacchromyces cerevisiae genome by inserting lacZ as a reporter gene under the control of two different promoters and determining expression levels through enzyme activity measurement. An up to 8.7-fold difference was detected between the sites conferring lowest and highest expression, respectively. This opens the opportunity for modulating gene expression levels while retaining promoter and culture conditions. Copyright (C) 2009 John Wiley & Sons, Ltd.

escherichia-coli

decarboxylase

specificity

solo LTRs

precursor

Saccharomyces cerevisiae

pathway

genome

sequence

lacZ

structural gene

beta-carotene

yeast

reporter gene

Author

D. B. Flagfeldt

Verena Siewers

L. Huang

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Yeast

0749-503X (ISSN) 1097-0061 (eISSN)

Vol. 26 10 545-551

Subject Categories

Industrial Biotechnology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1002/yea.1705

More information

Latest update

4/20/2018