The Influence of Microgravity on Invasive Growth in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2011

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Sigma 1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Sigma 1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Sigma 1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.

Proteomics

Microgravity

Adhesion

escherichia-coli

yeast

international-space-station

gene-expression

shear modeled microgravity

cell-cell

candida-albicans

biofilm formation

Invasive growth

Budding

stress-response

Saccharomyces cerevisiae

immune-system

Författare

S. E. Van Mulders

KU Leuven

C. Stassen

Universiteit Gent

L. Daenen

KU Leuven

B. Devreese

Universiteit Gent

Verena Siewers

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

R. G. E. van Eijsden

Flanders Interuniversity Institute for Biotechnology

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

F. R. Delvaux

KU Leuven

R. G. Willaert

Vrije Universiteit Brussel

Astrobiology

1531-1074 (ISSN)

Vol. 11 45-55

Ämneskategorier

Biologiska vetenskaper

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1089/ast.2010.0518