Reconstruction and Evaluation of the Synthetic Bacterial MEP Pathway in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2012

Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.

arabidopsis-thaliana

in-vivo

4fe-4s protein

escherichia-coli

deoxyxylulose phosphate-pathway

transfer-rnas

isoprenoid biosynthetic-pathway

sterol levels

isph protein

gene synthesis

Författare

Siavash Partow

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Verena Siewers

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

L. Daviet

Firmenich SA

M. Schalk

Firmenich SA

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

PLoS ONE

1932-6203 (ISSN)

Vol. 7 Article Number: e52498 - e52498

Fundament

Grundläggande vetenskaper

Ämneskategorier

Kemiteknik

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1371/journal.pone.0052498