Reconstruction and Evaluation of the Synthetic Bacterial MEP Pathway in Saccharomyces cerevisiae
Journal article, 2012

Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.

escherichia-coli

transfer-rnas

isph protein

arabidopsis-thaliana

in-vivo

sterol levels

deoxyxylulose phosphate-pathway

gene synthesis

4fe-4s protein

isoprenoid biosynthetic-pathway

Author

Siavash Partow

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

L. Daviet

Firmenich Inc.

M. Schalk

Firmenich Inc.

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

PLoS ONE

1932-6203 (ISSN)

Vol. 7 12 Article Number: e52498 - e52498

Roots

Basic sciences

Subject Categories

Chemical Engineering

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1371/journal.pone.0052498

More information

Latest update

9/6/2018 1