Harnessing yeast peroxisomes for biosynthesis of fatty-acid-derived biofuels and chemicals with relieved side-pathway competition
Artikel i vetenskaplig tidskrift, 2016

Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing pathways and/or insufficient precursor supply. Compartmentalization in cellular organelles can isolate synthetic pathways from competing pathways, and provide a compact and suitable environment for biosynthesis. Peroxisomes are cellular organelles where fatty acids are degraded, a process that is inhibited under typical fermentation conditions making them an interesting workhouse for production of fatty-acid-derived molecules. Here, we show that targeting synthetic pathways to peroxisomes can increase the production of fatty-acid-derived fatty alcohols, alkanes and olefins up to 700%. In addition, we demonstrate that biosynthesis of these chemicals in the peroxisomes results in significantly decreased accumulation of byproducts formed by competing enzymes. We further demonstrate that production can be enhanced up to 3-fold by increasing the peroxisome population. The strategies described here could be used for production of other chemicals, especially acyl-CoA-derived molecules.

Metabolic engineering

Fatty acid

Biofuels

Författare

Yongjin Zhou

Chalmers, Biologi och bioteknik, Systembiologi

Nicolaas Buijs

Chalmers, Biologi och bioteknik, Systembiologi

Zhu Zhiwei

Chalmers, Biologi och bioteknik, Systembiologi

Diego Orol Gómez

Chalmers, Biologi och bioteknik, Systembiologi

Akarin Boonsombuti

Chalmers, Biologi och bioteknik, Systembiologi

Verena Siewers

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 138 15368-15377

Infrastruktur

Chalmers infrastruktur för masspektrometri

Styrkeområden

Energi

Livsvetenskaper och teknik

Ämneskategorier

Bioenergi

Mikrobiologi

DOI

10.1021/jacs.6b07394