Harnessing yeast peroxisomes for biosynthesis of fatty-acid-derived biofuels and chemicals with relieved side-pathway competition
Journal article, 2016

Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing pathways and/or insufficient precursor supply. Compartmentalization in cellular organelles can isolate synthetic pathways from competing pathways, and provide a compact and suitable environment for biosynthesis. Peroxisomes are cellular organelles where fatty acids are degraded, a process that is inhibited under typical fermentation conditions making them an interesting workhouse for production of fatty-acid-derived molecules. Here, we show that targeting synthetic pathways to peroxisomes can increase the production of fatty-acid-derived fatty alcohols, alkanes and olefins up to 700%. In addition, we demonstrate that biosynthesis of these chemicals in the peroxisomes results in significantly decreased accumulation of byproducts formed by competing enzymes. We further demonstrate that production can be enhanced up to 3-fold by increasing the peroxisome population. The strategies described here could be used for production of other chemicals, especially acyl-CoA-derived molecules.

Metabolic engineering

Fatty acid

Biofuels

Author

Yongjin Zhou

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Nicolaas Buijs

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Zhu Zhiwei

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Diego Orol Gómez

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Akarin Boonsombuti

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Verena Siewers

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Journal of the American Chemical Society

0002-7863 (ISSN) 1520-5126 (eISSN)

Vol. 138 47 15368-15377

Infrastructure

Chalmers Infrastructure for Mass spectrometry

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Subject Categories

Bioenergy

Microbiology

DOI

10.1021/jacs.6b07394

More information

Created

10/8/2017