A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study
Artikel i vetenskaplig tidskrift, 2013

Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.

Saccharomyces cerevisiae

Fluxomics

RNAlater

Transcriptomics

Genomics

Omics

Författare

R. G. E. van Eijsden

Flanders Interuniversity Institute for Biotechnology

C. Stassen

Universiteit Gent

L. Daenen

KU Leuven

S. E. Van Mulders

KU Leuven

P. M. Bapat

Danmarks Tekniske Universitet (DTU)

Verena Siewers

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

K. V. Y. Goossens

Vrije Universiteit Brüssel (VUB)

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

F. R. Delvaux

KU Leuven

P. Van Hummelen

Dana-Farber Cancer Institute

B. Devreese

Universiteit Gent

R. G. Willaert

Vrije Universiteit Brüssel (VUB)

Biotechnology Letters

0141-5492 (ISSN) 1573-6776 (eISSN)

Vol. 35 891-900

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Bioinformatik och systembiologi

DOI

10.1007/s10529-013-1163-0