A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study
Journal article, 2013

Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.

Genomics

Omics

Fluxomics

RNAlater

Saccharomyces cerevisiae

Transcriptomics

Author

R. G. E. van Eijsden

Flanders Interuniversity Institute for Biotechnology

C. Stassen

Ghent university

L. Daenen

KU Leuven

S. E. Van Mulders

KU Leuven

P. M. Bapat

Technical University of Denmark (DTU)

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

K. V. Y. Goossens

Vrije Universiteit Brussel (VUB)

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

F. R. Delvaux

KU Leuven

P. Van Hummelen

Dana-Farber Cancer Institute

B. Devreese

Ghent university

R. G. Willaert

Vrije Universiteit Brussel (VUB)

Biotechnology Letters

0141-5492 (ISSN) 1573-6776 (eISSN)

Vol. 35 6 891-900

Areas of Advance

Life Science Engineering (2010-2018)

Subject Categories

Bioinformatics and Systems Biology

DOI

10.1007/s10529-013-1163-0

More information

Latest update

5/29/2018