Phosphoglycerate mutase knock-out mutant Saccharomyces cerevisiae: Physiological investigation and transcriptome analysis
Artikel i vetenskaplig tidskrift, 2010

The yeast Saccharomyces cerevisiae is able to adapt its metabolism to grow on different carbon sources and to shift to non-fermentative growth on C-2 or C-3 carbon sources (ethanol, acetate, or glycerol) through the activation of gluconeogenesis. Here, we studied the response to tine deletion of the glycolytic and gluconeogenic gene GPM1, encoding for phosphoglycerate mutase. It was previously shown that a S. cerevisiae strain with non-functional copies of GPM1 can only grow when glycerol and ethanol are both present as carbon sources, whilst addition of glucose was shown to strongly inhibit growth. It was suggested that glycerol is needed to feed gluconeogenesis whilst ethanol is required for respiration. Here, we studied the physiological response of the GPM1 knock-out mutant through fermentation and transcriptome analysis. Furthermore, we compared the physiological results with those obtained through simulations using a genome-scale metabolic model, showing that glycerol is only needed in small amounts for growth. Our findings strongly suggest a severely impaired growth ability of the knock-out mutant, which presents increased transcript levels of genes involved in the pentose phosphate pathway and in the glyoxylate shunt. These results indicate an attempt to compensate for the energy imbalance caused by the deletion of the glycolytic/gluconeogenic gene within the mutant.

Genome scale metabolic model

Trancriptome analysis

Glycerophosphomutase

Fermentation

Systems biology

Författare

Marta Papini

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Intawat Nookaew

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Gionata Scalcinati

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Verena Siewers

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)

Vol. 5 10 1016-1027

Ämneskategorier

Industriell bioteknik

Mikrobiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1002/biot.201000199