Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing
Artikel i vetenskaplig tidskrift, 2014

Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed for improving the ethanol yield while reducing glycerol production. Here we studied the effect of reducing glycerol export/formation through deletion of the aquaglyceroporin gene FPS1 together with expressing gapN encoding NADP+-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans and overexpressing the ATP-NADH kinase gene UTR1 from S. cerevisiae. This strategy will allow reducing the redox balance problem observed when the glycerol pathway is blocked, and hereby improve ethanol production. We found that our strategy enabled increasing the ethanol yield by 4.6% in the case of the best producing strain, compared to the reference strain, without any major effect on the specific growth rate.

Redox balancing

Ethanol production

Saccharomyces cerevisiae

Glycerol

Författare

Clara Navarrete Roman

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Verena Siewers

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

AMB Express

2191-0855 (ISSN)

Vol. 4 1 1-8

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Kemi

DOI

10.1186/s13568-014-0086-z

Mer information

Skapat

2017-10-07