Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing
Journal article, 2014

Ethanol is by volume the largest fermentation product. During ethanol production by Saccharomyces cerevisiae about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed for improving the ethanol yield while reducing glycerol production. Here we studied the effect of reducing glycerol export/formation through deletion of the aquaglyceroporin gene FPS1 together with expressing gapN encoding NADP+-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans and overexpressing the ATP-NADH kinase gene UTR1 from S. cerevisiae. This strategy will allow reducing the redox balance problem observed when the glycerol pathway is blocked, and hereby improve ethanol production. We found that our strategy enabled increasing the ethanol yield by 4.6% in the case of the best producing strain, compared to the reference strain, without any major effect on the specific growth rate.

Redox balancing

Ethanol production

Saccharomyces cerevisiae



Clara Navarrete Roman

Chalmers, Chemical and Biological Engineering, Life Sciences

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences

AMB Express

21910855 (eISSN)

Vol. 4 1 1-8 86

Areas of Advance

Life Science Engineering (2010-2018)

Subject Categories

Chemical Sciences



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4/5/2022 7