A novel qualitative and quantitative biofilm assay based on 3D soft tissue
Journal article, 2014
The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections. © 2014 Bodil Hakonen et al.
quantitative assay
device infection
wound dressing
qualitative assay
silver
controlled study
in vitro study
screening
agar
prediction
drug delivery device
antibiotic agent
in vivo study
bacterial load
antimicrobial activity
article
biofilm
nonhuman
assay
Pseudomonas aeruginosa
aquacel
mepilex
bacterial strain
unclassified drug
priority journal
soft tissue
bacterium culture
allevyn
Author
Bodil Hakonen
Linnea K. Lönnberg
University of Gothenburg
E. Larkö
K. Blom
International Journal of Biomaterials
1687-8787 (ISSN) 1687-8795 (eISSN)
Vol. 2014 Article ID 768136-DOI
10.1155/2014/768136