A novel qualitative and quantitative biofilm assay based on 3D soft tissue
Artikel i vetenskaplig tidskrift, 2014

The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.

unclassified drug

biofilm

bacterial strain

assay

Pseudomonas aeruginosa

mepilex

drug delivery device

silver

bacterium culture

agar

controlled study

qualitative assay

prediction

in vivo study

device infection

antibiotic agent

nonhuman

screening

bacterial load

quantitative assay

allevyn

antimicrobial activity

wound dressing

aquacel

priority journal

in vitro study

soft tissue

article

Författare

Bodil Hakonen

Fysikalisk kemi

Linnea K. Lönnberg

Göteborgs universitet

E. Larkö

Medibiome

K. Blom

Medibiome

International Journal of Biomaterials

1687-8787 (ISSN) 1687-8795 (eISSN)

Vol. 2014 Article ID 768136-

Ämneskategorier

Fysikalisk kemi

DOI

10.1155/2014/768136

Mer information

Senast uppdaterat

2024-05-24