Fast size-determination of intact bacterial plasmids using nanofluidic channels
Journal article, 2015
We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.
Author
Karolin Frykholm
Chalmers, Biology and Biological Engineering, Chemical Biology
Lena Nyberg
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
E. Lagerstedt
Lund University
C. Noble
Lund University
Joachim Fritzsche
Chalmers, Applied Physics, Chemical Physics
N. Karami
Sahlgrenska University Hospital
T. Ambjornsson
Lund University
L. Sandegren
Uppsala University
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
Lab on a Chip - Miniaturisation for Chemistry and Biology
1473-0197 (ISSN) 1473-0189 (eISSN)
Vol. 15 13 2739-2743Areas of Advance
Nanoscience and Nanotechnology
Life Science Engineering (2010-2018)
Subject Categories
Biochemistry and Molecular Biology
DOI
10.1039/c5lc00378d
PubMed
25997119