Isolation of novel IncA/C and IncN fluoroquinolone resistance plasmids from an antibiotic-polluted lake
Journal article, 2015

Objectives Antibiotic-polluted environments may function as reservoirs for novel resistance plasmids not yet encountered in pathogens. The aims of this study were to assess the potential of resistance transfer between bacteria from such environments and Escherichia coli, and to characterize the conjugative elements involved. Methods Sediment samples from Kazipally lake and Asanikunta tank, two Indian lakes with a history of severe pollution with fluoroquinolones, were investigated. Proportions of resistant bacteria were determined by selective cultivation, while horizontal gene transfer was studied using a GFP-tagged E. coli as recipient. Retrieved transconjugants were tested for susceptibility by Etest® and captured conjugative resistance elements were characterized by WGS. Results The polluted lakes harboured considerably higher proportions of ciprofloxacin-resistant and sulfamethoxazole-resistant bacteria than did other Indian and Swedish lakes included for comparison (52% versus 2% and 60% versus 7%, respectively). Resistance plasmids were captured from Kazipally lake, but not from any of the other lakes; in the case of Asanikunta tank because of high sediment toxicity. Eight unique IncA/C and IncN resistance plasmids were identified among 11 sequenced transconjugants. Five plasmids were fully assembled, and four of these carried the quinolone resistance gene qnrVC1, which has previously only been found on chromosomes. Acquired resistance genes, in the majority of cases associated with class 1 integrons, could be linked to decreased susceptibility to several different classes of antibiotics. Conclusions Our study shows that environments heavily polluted with antibiotics contain novel multiresistance plasmids transferrable to E. coli.

Author

Carl-Fredrik Flach

University of Gothenburg

Anna Johnning

University of Gothenburg

Chalmers, Mathematical Sciences, Mathematical Statistics

Ida Nilsson

University of Gothenburg

Kornelia Smalla

Institute for Epidemiology and Pathogen Diagnostics

Erik Kristiansson

Chalmers, Mathematical Sciences, Mathematical Statistics

University of Gothenburg

D. G. Joakim Larsson

University of Gothenburg

Journal of Antimicrobial Chemotherapy

0305-7453 (ISSN) 1460-2091 (eISSN)

Vol. 70 10 2709-2717

Characterization of antibiotic resistance in bacterial communities: novel methods using next generation sequencing

Swedish Research Council (VR), 2012-01-01 -- 2015-12-31.

Subject Categories

Microbiology

Microbiology in the medical area

Bioinformatics and Systems Biology

DOI

10.1093/jac/dkv167

More information

Created

10/8/2017