Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae
Journal article, 2015

Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can convert both C6 and C5 sugars present in lignocellulosic biomass into the products of interest. We engineered Saccharomyces cerevisiae for production of 3-hydroxypropionic acid (3HP), a potential building block for acrylates, from glucose and xylose. We introduced the 3HP biosynthetic pathways via malonyl-CoA or β-alanine intermediates into a xylose-consuming yeast. Using controlled fed-batch cultivation, we obtained 7.37±0.17g 3HPL-1 in 120hours with an overall yield of 29±1%Cmol 3HPCmol-1 xylose. This study is the first demonstration of the potential of using S. cerevisiae for production of 3HP from the biomass sugar xylose.

Xylose utilization

Metabolic engineering


3-hydroxypropionic acid

Saccharomyces cerevisiae


K. R. Kildegaard

Technical University of Denmark (DTU)

Zheng Wang

Chalmers, Biology and Biological Engineering

Yun Chen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

I. Borodina

Technical University of Denmark (DTU)

Metabolic Engineering Communications

2214-0301 (eISSN)

Vol. 2 132-136

Areas of Advance


Life Science Engineering (2010-2018)

Subject Categories

Bioinformatics and Systems Biology



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