Engineering Cellular Metabolism
Review article, 2016

Metabolic engineering is the science of rewiring the metabolism of cells to enhance production of native metabolites or to endow cells with the ability to produce new products. The potential applications of such efforts are wide ranging, including the generation of fuels, chemicals, foods, feeds, and pharmaceuticals. However, making cells into efficient factories is challenging because cells have evolved robust metabolic networks with hard-wired, tightly regulated lines of communication between molecular pathways that resist efforts to divert resources. Here, we will review the current status and challenges of metabolic engineering and will discuss how new technologies can enable metabolic engineering to be scaled up to the industrial level, either by cutting off the lines of control for endogenous metabolism or by infiltrating the system with disruptive, heterologous pathways that overcome cellular regulation.

de-novo production

high-level production




Biochemistry & Molecular Biology

Cell Biology

synthetic biology


acetyl-coa metabolism


genome-scale models

regulatory network


Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

J.D. Keasling

Technical University of Denmark (DTU)

Synthetic Biology Engineering Research Center (SynBERC)

Joint BioEnergy Institute, California

University of California


0092-8674 (ISSN) 1097-4172 (eISSN)

Vol. 164 6 1185-1197

Subject Categories

Biochemistry and Molecular Biology

Areas of Advance


Life Science Engineering (2010-2018)



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7/2/2021 6