Diastereomeric bactericidal effect of Ru(phenanthroline)(2)dipyridophenazine
Journal article, 2016

Metal susceptibility assays and spot plating were used to investigate the antimicrobial activity of enantiopure [Ru(phen)(2)dppz](2+) (phen =1,10-phenanthroline and dppz = dipyrido[3,2-a:2 ',3 '-c]phenazine) and [-bidppz(phen)(4)Ru-2](4+) (bidppz =11,11 '-bis(dipyrido[3,2-a:2 ',3 '-c]phenazinyl)), on Gram-negative Escherichia coli and Gram-positive Bacillus subtilis as bacterial models. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined for both complexes: while [-bidppz(phen)(4)Ru-2](4+) only showed a bactericidal effect at the highest concentrations tested, the antimicrobial activity of [Ru(phen)(2)dppz](2+) against B. subtilis was comparable to that of tetracyline. In addition, the -enantiomer of [Ru(phen)(2)dppz](2+) showed a 2-fold higher bacteriostatic and bactericidal effect compared to the -enantiomer. This was in accordance with the enantiomers relative binding affinity for DNA, thus strongly indicating DNA binding as the mode of action.

ruthenium complex

antimicrobial activity

emission

chirality

metal susceptibility assay

confocal microscopy

minimum inhibitory concentration

Author

Anna Mårtensson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Mattias Bergentall

University of Gothenburg

Valentina Tremaroli

University of Gothenburg

Per Lincoln

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Chirality

0899-0042 (ISSN) 1520-636X (eISSN)

Vol. 28 11 713-720

Areas of Advance

Nanoscience and Nanotechnology

Life Science Engineering

Subject Categories

Biochemistry and Molecular Biology

Microbiology

DOI

10.1002/chir.22656

More information

Created

10/8/2017