Diastereomeric bactericidal effect of Ru(phenanthroline)(2)dipyridophenazine
Artikel i vetenskaplig tidskrift, 2016

Metal susceptibility assays and spot plating were used to investigate the antimicrobial activity of enantiopure [Ru(phen)(2)dppz](2+) (phen =1,10-phenanthroline and dppz = dipyrido[3,2-a:2 ',3 '-c]phenazine) and [-bidppz(phen)(4)Ru-2](4+) (bidppz =11,11 '-bis(dipyrido[3,2-a:2 ',3 '-c]phenazinyl)), on Gram-negative Escherichia coli and Gram-positive Bacillus subtilis as bacterial models. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined for both complexes: while [-bidppz(phen)(4)Ru-2](4+) only showed a bactericidal effect at the highest concentrations tested, the antimicrobial activity of [Ru(phen)(2)dppz](2+) against B. subtilis was comparable to that of tetracyline. In addition, the -enantiomer of [Ru(phen)(2)dppz](2+) showed a 2-fold higher bacteriostatic and bactericidal effect compared to the -enantiomer. This was in accordance with the enantiomers relative binding affinity for DNA, thus strongly indicating DNA binding as the mode of action.

ruthenium complex

antimicrobial activity

emission

chirality

metal susceptibility assay

confocal microscopy

minimum inhibitory concentration

Författare

Anna Mårtensson

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Mattias Bergentall

Göteborgs universitet

Valentina Tremaroli

Göteborgs universitet

Per Lincoln

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Chirality

0899-0042 (ISSN) 1520-636X (eISSN)

Vol. 28 11 713-720

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

DOI

10.1002/chir.22656