D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
Journal article, 2012

d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

d-Lactate dehydrogenase

Arabidopsis thaliana

Plant transformation

Selection marker

Author

J. Wienstroer

Heinrich Heine University Düsseldorf

Martin Engqvist

University of Cologne

California Institute of Technology (Caltech)

H.H. Kunz

University of Cologne

U.I. Flügge

University of Cologne

V.G. Maurino

Heinrich Heine University Düsseldorf

University of Cologne

FEBS Letters

0014-5793 (ISSN)

Vol. 586 1 36-40

Subject Categories

Botany

Biochemistry and Molecular Biology

DOI

10.1016/j.febslet.2011.11.020

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Latest update

4/9/2020 1