D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
Artikel i vetenskaplig tidskrift, 2012

d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

Plant transformation

Arabidopsis thaliana

d-Lactate dehydrogenase

Selection marker

Författare

J. Wienstroer

Martin Engqvist

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

H.H. Kunz

U.I. Flügge

V.G. Maurino

FEBS Letters

0014-5793 (ISSN)

Vol. 586 1 36-40

Ämneskategorier

Botanik

Biokemi och molekylärbiologi

DOI

10.1016/j.febslet.2011.11.020

Mer information

Skapat

2017-10-07