D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
Artikel i vetenskaplig tidskrift, 2012

d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

d-Lactate dehydrogenase

Arabidopsis thaliana

Plant transformation

Selection marker

Författare

J. Wienstroer

Heinrich Heine Universität Düsseldorf

Martin Engqvist

Universität zu Köln

California Institute of Technology (Caltech)

H.H. Kunz

Universität zu Köln

U.I. Flügge

Universität zu Köln

V.G. Maurino

Heinrich Heine Universität Düsseldorf

Universität zu Köln

FEBS Letters

0014-5793 (ISSN)

Vol. 586 1 36-40

Ämneskategorier

Botanik

Biokemi och molekylärbiologi

DOI

10.1016/j.febslet.2011.11.020

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Senast uppdaterat

2020-04-09