Develpoment of a Simplified Method for the Determination of Folates in BakerĀ“s Yeastby HPLC with Ultraviolet and Fluorescence Detection
Journal article, 2005

A simplified HPLC method for rapid determination of folates in yeast with ultraviolet and fluorescence detection without sample purification has been developed. By use of the column Aquasil C-18, specially designed for polar analytes, and gradient elution, it was possible to separate and determine five folate derivatives: tetrahydrofolate, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate with fluorescence detection, and 10-formylfolic acid and folic acid with ultraviolet detection. The sample preparation required only a small amount of dry yeast (25-50 mg) and included an extraction of folates by heat treatment and deconjugation of folate polyglutamates to monoglutamates with the use of rat serum conjugase. Validation involved investigation of matrix effects, determination of recovery by standard addition method, repeatability, and stability tests. The dominating folate forms in commercial dry baker's yeast were found to be tetrahydrafolate and 5-methyltetrahydrofolate with a total folate content of 2890 mu g/100 g (63.4 nmol/g). The simplicity of the method makes it suitable for folate screening studies of different yeast strains.

Author

Johan Patring

Swedish University of Agricultural Sciences (SLU)

Jelena Jastrebova

Swedish University of Agricultural Sciences (SLU)

Sofia Hjortmo

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Thomas Andlid

Chalmers, Chemical and Biological Engineering, Life Sciences, Food and Nutrition Science

Margaretha Jägerstad

Swedish University of Agricultural Sciences (SLU)

Journal of Agricultural and Food Chemistry

0021-8561 (ISSN) 1520-5118 (eISSN)

Vol. 53 7 2406-2411

Subject Categories

Other Industrial Biotechnology

DOI

10.1021/jf048083g

More information

Latest update

4/11/2018