Metabolic engineering of photorespiration
Book chapter, 2017

The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.

Arabidopsis transformation

Agrobacterium tumefaciens

PCR amplification

Binary vectors

Author

Martin Engqvist

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

V.G. Maurino

Heinrich Heine University Düsseldorf

Methods in Molecular Biology

10643745 (ISSN)

137-155

Subject Categories

Microbiology

DOI

10.1007/978-1-4939-7225-8_10

More information

Created

10/8/2017