Metabolic engineering of photorespiration
Kapitel i bok, 2017

The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.

Arabidopsis transformation

Agrobacterium tumefaciens

PCR amplification

Binary vectors

Författare

Martin Engqvist

Chalmers, Biologi och bioteknik, Systembiologi

V.G. Maurino

Heinrich Heine Universitat

Methods in molecular biology (Clifton, N.J.)

1064-3745 (ISSN)

137-155

Ämneskategorier

Mikrobiologi

DOI

10.1007/978-1-4939-7225-8_10