Competitive DNA binding of Ru(bpy)2dppz2+ enantiomers studied with isothermal titration calorimetry (ITC) using a direct and general binding isotherm algorithm
Journal article, 2018

While isothermal titration calorimetry (ITC) is widely used and sometimes referred to as the “gold standard” for quantitative measurements of biomolecular interactions, its usage has so far been limited to the analysis of the binding to isolated, non-cooperative binding sites. Studies on more complicated systems, where the binding sites interacts, causing either cooperativity or anti-cooperativity between neighboring bound ligands, are rare, probably due to the complexity of the methods currently available. Here we have developed a simple algorithm not limited by the complexity of a binding system, meaning that it can be implemented by anyone, from analyzing systems of simple, isolated binding sites to complicated interactive multiple-site systems. We demonstrate here that even complicated competitive binding calorimetric isotherms can be properly analyzed, provided that ligand-ligand interactions are taken into account. As a practical example, the competitive binding interactions between the two enantiomers of Ru(bpy)2dppz²⁺ (Ru-bpy) and poly(dAdT)2 (AT-DNA) is analyzed using our new algorithm, which provided an excellent global fit for the ITC experimental data.

Author

Anna Mårtensson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Per Lincoln

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Physical Chemistry Chemical Physics

1463-9076 (ISSN) 1463-9084 (eISSN)

Vol. 20 12 7920-7930

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Subject Categories

Physical Chemistry

Biophysics

Roots

Basic sciences

DOI

10.1039/c7cp03184j

More information

Latest update

5/9/2018 2