Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition
Journal article, 2018

Background: Cannabis saliva(hemp) is a source of various biologically active compounds, for instance, cannabinoids, terpenes and phenolic compounds, which exhibit antibacterial, antifungal, anti-inflammatory and anticancer properties. With the purpose of expanding the auxiliary application of C. sativa in the field of bio-nanotechnology, we explored the plant for green and efficient synthesis of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). Methods and results: The nanoparticles were synthesized by utilizing an aqueous extract of C. sativa stem separated into two different fractions (cortex and core [xylem part]) without any additional reducing, stabilizing and capping agents. In the synthesis of AuNPs using the cortex enriched in bast fibers, fiber-AuNPs (F-AuNPs) were achieved. When using the core part of the stem, which is enriched with phenolic compounds such as alkaloids and cannabinoids, core-AuNPs (C-AuNPs) and core-AgNPs (C-AgNPs) were formed. Synthesized nanoparticles were characterized by UV-visible analysis, transmission electron microscopy, atomic force microscopy, dynamic light scattering, Fourier transform infrared, and matrix-assisted laser desorption/ionization timeof-flight. In addition, the stable nature of nanoparticles has been shown by thermogravimetric analysis and inductively coupled plasma mass spectrometry (ICP-MS). Finally, the AgNPs were explored for the inhibition of Pseudomonas aeruginosa and Escherichia coli biofilms. Condusion: The synthesized nanoparticles were crystalline with an average diameter between 12 and 18 nm for F-AuNPs and C-AuNPs and in the range of 20-40 nm for C-AgN Ps. ICP-MS analysis revealed concentrations of synthesized nanoparticles as 0.7, 4.5 and 3.6 mg/mL for F-AuNPs, C-AuNPs and C-AgNPs, respectively. Fourier transform infrared spectroscopy revealed the presence of flavonoids, cannabinoids, terpenes and phenols on the nanoparticle surface, which could be responsible for reducing the salts to nanoparticles and further stabilizing them. In addition, the stable nature of synthesized nanoparticles has been shown by thermogravimetric analysis and ICP-MS. Finally, the AgNPs were explored for the inhibition of P. aeruginosa and E. coli biofilms. The nanoparticles exhibited minimum inhibitory concentration values of 6.25 and 5 mu g/mL and minimum bactericidal concentration values of 12.5 and 25 mu g/mL against P. aeruginosa and E. coil, respectively.

pathogenic strains

biofilm

hemp

ICP-MS

gold nanoparticles

medicinal plant

Cannabis sativa

nanotechnology

nanoparticle concentration

biomedical application

silver nanoparticles

Author

[Person 24f831ff-7cd1-4101-bed6-229249355db6 not found]

Technical University of Denmark (DTU)

[Person 2a179336-e3c4-4755-98a3-776bcec477e0 not found]

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

[Person f133a6b6-21a7-48ad-880e-147241755a23 not found]

Danish Fundamental Metrology

[Person 95e1a349-a3d0-42c1-94c7-92b59cdb8e13 not found]

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

[Person 46f5995f-a9b2-4d18-99f7-b2ad64e14be5 not found]

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

[Person 83adaeeb-0e65-4fef-b017-c71bfc921ba6 not found]

Technical University of Denmark (DTU)

[Person db650556-1bb7-44a0-b322-b7391ca66fb7 not found]

Technical University of Denmark (DTU)

[Person b5781443-609c-449a-bb92-da4af8111988 not found]

Technical University of Denmark (DTU)

[Person 4f4ab2ef-cfdf-431a-a03a-1bc69a8fb48b not found]

Technical University of Denmark (DTU)

[Person c903021a-a3d0-4b55-b938-b473b8075a8f not found]

Technical University of Denmark (DTU)

[Person e784cb32-241b-4ca5-bc62-bfcda261bcf8 not found]

Technical University of Denmark (DTU)

[Person 2118e67f-adfb-4178-bacb-98192b435710 not found]

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

International journal of nanomedicine

1176-9114 (ISSN) 1178-2013 (eISSN)

Vol. 13 3571-3591

Subject Categories

Analytical Chemistry

Materials Chemistry

Other Chemistry Topics

DOI

10.2147/IJN.S157958

More information

Latest update

9/18/2018