Plasma cells and Fc receptors in human adipose tissue--lipogenic and anti-inflammatory effects of immunoglobulins on adipocytes
Journal article, 2006
We have previously reported high immunoglobulin expression in human omental adipose tissue. The aim of this work was to investigate plasma cell density and Fc receptor (FcR) expression in human adipose tissue depots and in vitro effects of immunoglobulins on adipocyte function. Plasma cell density was higher in the visceral compared to the subcutaneous depot (10.0+/-1.56% and 5.2+/-0.98%, respectively, n=20, p<0.05). Microarray analysis revealed expression of four FcR genes in adipose tissue; FCGR2A, FCGR2B, FCER1G, and FCGRT. FCGR2A was highly expressed in adipocytes in both depots and this was verified by immunohistochemistry. Expression of IL-1beta and IL-6 was markedly reduced in adipocytes after incubation with the Fc moiety of immunoglobulin G (Fc) (p<0.01). Furthermore, Fc stimulated adipocyte lipogenesis as potently as insulin (p<0.05), but did not influence lipolysis. In conclusion, immunoglobulins produced by plasma cells in human adipose tissue could influence adipocyte metabolism and cytokine production.
Adipocytes/immunology
Male
Humans
Female
Adipose Tissue/*cytology/*immunology
Plasma Cells/*immunology
Omentum/cytology
Fc/*analysis/*genetics
Adult
Cell Count
Gene Expression
Cytokines/genetics
Oligonucleotide Array Sequence Analysis
Lipid Metabolism/immunology
Immunoglobulins/*genetics
Receptors
Author
Jenny Palming
University of Gothenburg
Britt Gabrielsson
University of Gothenburg
Eva Jennische
University of Gothenburg
Ulf Smith
University of Gothenburg
Björn Carlsson
University of Gothenburg
Lena M S Carlsson
University of Gothenburg
Malin Lönn
University of Gothenburg
Biochemical and Biophysical Research Communications
0006-291X (ISSN) 1090-2104 (eISSN)
Vol. 343 1 43-8Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.bbrc.2006.02.114
PubMed
16527253