Strain-dependent variance in short-term adaptation effects of two xylose-fermenting strains of Saccharomyces cerevisiae
Journal article, 2019

The limited tolerance of Saccharomyces cerevisiae to the inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. The aim of this study was to investigate the short-term adaptation effects in yeast strains with different genetic backgrounds. Fed-batch propagation cultures were supplemented with 40% wheat straw hydrolysate during the feed phase to adapt two different pentose-fermenting strains, CR01 and KE6-12. The harvested cells were used to inoculate fermentation media containing 80% or 90% wheat straw hydrolysate. The specific ethanol productivity during fermentation was up to 3.6 times higher for CR01 and 1.6 times higher for KE6-12 following adaptation. The influence of physiological parameters such as viability, storage carbohydrate content, and metabolite yields following short-term adaptation demonstrated that short-term adaptation was strain dependent.

Short-term adaptation


Wheat straw hydrolysate

Industrial Saccharomyces cerevisiae strains



Marlous van Dijk

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Borbála Erdei

Lund University

Mats Galbe

Lund University

Yvonne Nygård

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Bioresource technology

09608524 (ISSN) 18732976 (eISSN)

Vol. 292 121922- 121922

Bottlenecks in cellulosic ethanol production: xylose fermentation and cell propagation

Swedish Energy Agency (2015-006983), 2016-01-01 -- 2019-12-31.

Subject Categories


Biocatalysis and Enzyme Technology

Other Industrial Biotechnology





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