Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature
Journal article, 2019

Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis). However, the total protein yield was low, and to improve this, the effects of adding ulvan lyase, preincubating the seaweed homogenate at pH 8.5 and using different protein extraction temperatures (8 °C, RT and 40 °C), were evaluated in this study. Addition of ulvan lyase reduced protein solubility but increased the precipitation. Incubation at pH 8.5, without ulvan lyase added, significantly increased both protein solubility and precipitation at 8 °C and RT. Temperature per se had no effect on protein solubility, while protein precipitation increased with decreasing temperature. Highest protein yield (29%) was achieved when keeping the process at 8 °C with a preincubation step at pH 8.5 for 1 h. By these process modifications, the yield was 3.2 times higher than achieved by the control process (9.2%).

Protein extraction

Ulvan lyase

Protein yield

pH-shift

Seaweed

Ulva lactuca

Author

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Chalmers, Biology and Biological Engineering, Food and Nutrition Science

[Person 3af31757-8422-4fce-9412-a76e377558f4 not found]

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

[Person dffcab4b-1dd0-4716-a6a9-a7d2095f063b not found]

University of Gothenburg

[Person bb48d65a-d4dd-4e03-87e3-9457a4fb1807 not found]

University of Gothenburg

[Person 124ee3c3-efcd-4848-9512-7ba3b9fbaaa2 not found]

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

[Person 0f400a0e-2f67-46b9-a066-01f373552732 not found]

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

ACS Sustainable Chemistry & Engineering

2168-0485 (eISSN)

Vol. 7 15 12688-12691

Seaweed production systems with high value applications

Swedish Foundation for Strategic Research (SSF), 2015-01-01 -- 2019-12-31.

Driving Forces

Sustainable development

Subject Categories

Food Engineering

Structural Biology

Other Industrial Biotechnology

DOI

10.1021/acssuschemeng.9b02781

More information

Latest update

10/21/2019