Nanofluidic optical DNA mapping for rapid identification of antibiotics resistant plasmids
Paper in proceedings, 2018

Bacterial resistance to antibiotics has become a major threat to health worldwide. In 2014, the World Health Organization (WHO) classified antibiotic resistance as one of the major threats to human health.1 As the resistance genes are frequently transmitted via mobile genetic elements, such as plasmids, a method to rapidly detect and identify plasmids is needed. This paper reports an improved nanofluidic optical mapping strategy to identify plasmids and locate the antibiotic resistance genes via the Cas9/CRISPR technique. We used the assay to analyze clinical samples from a potential nosocomial outbreak in an Ethiopian hospital, tracing the transmission routes among wards.

Optical DNA mapping

Plasmids

Antibiotic resistance

Nosocomial outbreak

Author

Yii Lih Lin

Chalmers, Biology and Biological Engineering, Chemical Biology

Tsegaye Sewunet

Jimma University

Karolinska Institutet

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Christian G. Giske

Karolinska Institutet

Karolinska University Hospital

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Vol. 3 1822-1825

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Kaohsiung, Taiwan,

Subject Categories

Infectious Medicine

Microbiology

Medical Genetics

More information

Latest update

3/16/2020