Comprehensive proteomic investigation of infectious and inflammatory changes in late preterm prelabour rupture of membranes
Journal article, 2020

Preterm prelabour rupture of membranes beyond the 34th week of gestation (late PPROM) is frequently associated with the risk of the microbial invasion of the amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover amniotic fluid proteome response to the presence of MIAC and HCA in late PPROM. Protein dysregulation was associated with only five cases in the group of 15 women with confirmed MIAC and HCA. Altogether, 138 amniotic fluid proteins were changed in these five cases exclusively. These proteins were particularly associated with excessive neutrophil responses to infection, such as neutrophil degranulation and extracellular trap formation. We believe that the quantification of these proteins in amniotic fluid may assist in revealing women with the highest risk of excessive inflammatory response in late PPROM.

Author

M. Vajrychova

University Hospital Hradec Kralove

Jaroslav Stráník

University Hospital Hradec Kralove

Kristýna Pimková

Charles University

University Hospital Hradec Kralove

Malin Barman

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Rudolf Kukla

University Hospital Hradec Kralove

Petra Zedníková

University of Pardubice

Czech Academy of Sciences

University Hospital Hradec Kralove

Radka Bolehovská

University Hospital Hradec Kralove

Lenka Plíšková

University Hospital Hradec Kralove

Helena Hornychová

University Hospital Hradec Kralove

Ctirad Andrýs

University Hospital Hradec Kralove

V. Tambor

University Hospital Hradec Kralove

J. Lenco

University Hospital Hradec Kralove

Charles University

Bo Jacobsson

Norwegian Institute of Public Health

University of Gothenburg

Marian Kacerovský

University Hospital Hradec Kralove

Charles University

Scientific Reports

2045-2322 (ISSN)

Vol. 10 1 17696

Subject Categories

Infectious Medicine

Microbiology in the medical area

Obstetrics, Gynecology and Reproductive Medicine

DOI

10.1038/s41598-020-74756-9

PubMed

33077789

More information

Latest update

11/16/2020