Developing an optical DNA mapping toolbox to identify chromosomal translocations in acute myeloid leukemia
Paper in proceedings, 2019

We present a method based on CRISPR/Cas9 technology and optical DNA mapping (ODM) for clinical diagnosis of chromosomal translocations in Acute Myeloid Leukemia. CRISPR/Cas9 is used to cut-out genomic regions of interest, which are then differentially stained, stretched inside nanochannels and imaged. Sequence-dependent intensity pattern of stretched molecules enable studying linear association of genomic regions and pinpoint structural variations. Our method can reveal DNA structural details on length-scales that are difficult to address by state-of-the-art molecular diagnostic approaches. This manuscript reports proof-of-concept results for CRISPR/Cas9 excision of a fragment in the E.coli genome and high-fidelity mapping of the human genome.

CRISPR/Cas9

Nanochannels

Optical DNA mapping

Acute Myeloid Leukemia

Author

Miriam Hitz

Chalmers, Biology and Biological Engineering, Chemical Biology

Fachhochschule Aachen

Gaurav Goyal

Chalmers, Biology and Biological Engineering, Chemical Biology

Vilhelm Müller

Chalmers, Biology and Biological Engineering, Chemical Biology

Linda Fogelstrand

Sahlgrenska University Hospital

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

626-627

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Subject Categories

Bioinformatics (Computational Biology)

Medical Genetics

Genetics

More information

Latest update

11/13/2020