Developing an optical DNA mapping toolbox to identify chromosomal translocations in acute myeloid leukemia
Paper i proceeding, 2019

We present a method based on CRISPR/Cas9 technology and optical DNA mapping (ODM) for clinical diagnosis of chromosomal translocations in Acute Myeloid Leukemia. CRISPR/Cas9 is used to cut-out genomic regions of interest, which are then differentially stained, stretched inside nanochannels and imaged. Sequence-dependent intensity pattern of stretched molecules enable studying linear association of genomic regions and pinpoint structural variations. Our method can reveal DNA structural details on length-scales that are difficult to address by state-of-the-art molecular diagnostic approaches. This manuscript reports proof-of-concept results for CRISPR/Cas9 excision of a fragment in the E.coli genome and high-fidelity mapping of the human genome.

CRISPR/Cas9

Nanochannels

Optical DNA mapping

Acute Myeloid Leukemia

Författare

Miriam Hitz

Chalmers, Biologi och bioteknik, Kemisk biologi

Fachhochschule Aachen

Gaurav Goyal

Chalmers, Biologi och bioteknik, Kemisk biologi

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

Linda Fogelstrand

Sahlgrenska universitetssjukhuset

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

626-627

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Ämneskategorier

Bioinformatik (beräkningsbiologi)

Medicinsk genetik

Genetik

Mer information

Senast uppdaterat

2020-11-13