Optical DNA mapping using nanochannels for identification of plasmids carrying carbapenemase blandm-1 gene from patients admitted to a Vietnamese hospital
Paper in proceedings, 2019

Antibiotic misuse has resulted in a rapidly increasing prevalence of multidrug resistant (MDR) bacteria, posing serious threat to human health. Bacterial plasmids play a significant role in spreading antibiotic resistance genes between strains and species. Rapid identification and characterization of plasmids, and knowledge about the presence of resistance genes, thus becomes essential. Here, we use optical DNA mapping (ODM), to identify the plasmid carrying blaNDM-1 gene in samples collected from patients in Vietnam National Children's Hospital (VNCH) in Hanoi, Veitnam, and investigate if a single plasmid is responsible for spreading of the blaNDM-1 gene across different strains of K. pneumonia.

Optical DNA mapping

Plasmids

K. pneumoniae

Antibiotic resistance

Nanofluidics

Author

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Maud Nilsson

Linköping University

Björn Berglund

Linköping University

Linus Olson

Karolinska Institutet

Hoang Bich Ngoc

Vietnam National Children's Hospital

Tran Minh Dien

Vietnam National Children's Hospital

Mattias Larsson

Karolinska Institutet

Håkan Hanberger

Linköping University

Christian G. Giske

Karolinska Institutet

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

805-806

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Subject Categories

Infectious Medicine

Microbiology

Microbiology in the medical area

Areas of Advance

Health Engineering

More information

Latest update

11/13/2020