Protein kinase A controls yeast growth in visible light
Journal article, 2020

Background: A wide variety of photosynthetic and non-photosynthetic species sense and respond to light, having developed protective mechanisms to adapt to damaging effects on DNA and proteins. While the biology of UV light-induced damage has been well studied, cellular responses to stress from visible light (400–700 nm) remain poorly understood despite being a regular part of the life cycle of many organisms. Here, we developed a high-throughput method for measuring growth under visible light stress and used it to screen for light sensitivity in the yeast gene deletion collection. Results: We found genes involved in HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications of the translational elongation factor eEF2, and the oxidative stress response to be required for light resistance. Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. We therefore used an ectopic fluorescent PKA reporter and mutants with constitutively altered PKA activity to show that repression of PKA is essential for resistance to visible light. Conclusion: We conclude that yeast photobiology is multifaceted and that protein kinase A plays a key role in the ability of cells to grow upon visible light exposure. We propose that visible light impacts on the biology and evolution of many non-photosynthetic organisms and have practical implications for how organisms are studied in the laboratory, with or without illumination.

Genome-wide screen

Light sensitivity

Visible light

Yeast

Diphthamide modification

HOG signaling

Protein kinase A

Author

Mikael Molin

University of Gothenburg

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Katarina Logg

University of Gothenburg

Chalmers, Physics, CMAL

Kristofer Bodvard

Chalmers, Physics, Bionanophotonics

K. Peeters

University of Gothenburg

Annabelle Forsmark

University of Gothenburg

F. Roger

University of Gothenburg

Anna Jorhov

University of Gothenburg

Neha Mishra

University of Gothenburg

Horizon Discovery

Jean Marc Billod

University of Gothenburg

Bio-Me A/S

Sabiha Amir

University of Gothenburg

Mikael Andersson

University of Gothenburg

Leif A Eriksson

University of Gothenburg

Jonas Warringer

University of Gothenburg

Mikael Käll

Chalmers, Physics, Nano and Biophysics

Anders Blomberg

University of Gothenburg

BMC Biology

1741-7007 (ISSN)

Vol. 18 1 168

Stark plasmon-exciton koppling för effektiva foton-foton interaktioner

Swedish Research Council (VR), 2018-01-01 -- 2021-12-31.

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1186/s12915-020-00867-4

PubMed

33198745

More information

Latest update

12/1/2020