Protein kinase A controls yeast growth in visible light
Journal article, 2020
Background: A wide variety of photosynthetic and non-photosynthetic species sense and respond to light, having developed protective mechanisms to adapt to damaging effects on DNA and proteins. While the biology of UV light-induced damage has been well studied, cellular responses to stress from visible light (400–700 nm) remain poorly understood despite being a regular part of the life cycle of many organisms. Here, we developed a high-throughput method for measuring growth under visible light stress and used it to screen for light sensitivity in the yeast gene deletion collection. Results: We found genes involved in HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications of the translational elongation factor eEF2, and the oxidative stress response to be required for light resistance. Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. We therefore used an ectopic fluorescent PKA reporter and mutants with constitutively altered PKA activity to show that repression of PKA is essential for resistance to visible light. Conclusion: We conclude that yeast photobiology is multifaceted and that protein kinase A plays a key role in the ability of cells to grow upon visible light exposure. We propose that visible light impacts on the biology and evolution of many non-photosynthetic organisms and have practical implications for how organisms are studied in the laboratory, with or without illumination.
Light sensitivity
HOG signaling
Genome-wide screen
Protein kinase A
Yeast
Diphthamide modification
Visible light
Author
Mikael Molin
University of Gothenburg
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Katarina Logg
Chalmers, Physics, CMAL
University of Gothenburg
Kristofer Bodvard
Chalmers, Physics, Bionanophotonics
K. Peeters
University of Gothenburg
Annabelle Forsmark
University of Gothenburg
F. Roger
University of Gothenburg
Anna Jorhov
University of Gothenburg
Neha Mishra
University of Gothenburg
Horizon Discovery
Jean Marc Billod
University of Gothenburg
Bio-Me A/S
Sabiha Amir
University of Gothenburg
Mikael Andersson
University of Gothenburg
Leif A Eriksson
University of Gothenburg
Jonas Warringer
University of Gothenburg
Mikael Käll
Chalmers, Physics, Nano and Biophysics
Anders Blomberg
University of Gothenburg
BMC Biology
1741-7007 (eISSN)
Vol. 18 1 168Stark plasmon-exciton koppling för effektiva foton-foton interaktioner
Swedish Research Council (VR) (2017-04545), 2018-01-01 -- 2021-12-31.
Subject Categories
Biochemistry and Molecular Biology
Other Physics Topics
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
DOI
10.1186/s12915-020-00867-4
PubMed
33198745