Novel clearance of muscle proteins by muscle cells
Journal article, 2020

Blood levels of cardiac troponins (cTn) and myoglobin are analysed when myocardial infarction (MI) is suspected. Here we describe a novel clearance mechanism for muscle proteins by muscle cells. The complete plasma clearance profile of cTn and myoglobin was followed in rats after intravenous or intermuscular injections and analysed by PET and fluorescence microscopy of muscle biopsies and muscle cells. Compared with intravenous injections, only 5 % of cTnT, 0.6 % of cTnI and 8 % of myoglobin were recovered in the circulation following intramuscular injection. In contrast, 47 % of the renal filtration marker FITC-sinistrin and 81 % of cTn fragments from MI-patients were recovered after intramuscular injection. In addition, PET and biopsy analysis revealed that cTn was taken up by the quadriceps muscle and both cTn and myoglobin were endocytosed by cultured muscle cells. This local clearance mechanism could possibly be the dominant clearance mechanism for cTn, myoglobin and other muscle damage biomarkers released by muscle cells.

Muscle

Myoglobin

Clearance

Troponin

Endocytosis

Author

Aida Muslimovic

Sahlgrenska University Hospital

Vincent Fridén

Sahlgrenska University Hospital

Karin Starnberg

Sahlgrenska University Hospital

Olav Tenstad

University of Bergen

Heidi Espedal

University of Bergen

Kristina Vukusic

Sahlgrenska University Hospital

Susanne Nyström

Sahlgrenska University Hospital

Emelie Vilhelmsson Wesén

Chalmers, Biology and Biological Engineering, Chemical Biology

Elin Esbjörner Winters

Chalmers, Biology and Biological Engineering, Chemical Biology

Kristoffer Granholm

Sahlgrenska University Hospital

Bertil Lindahl

Akademiska Sjukhuset

Ola Hammarsten

Sahlgrenska University Hospital

European Journal of Cell Biology

0171-9335 (ISSN)

Vol. 99 8 151127

Subject Categories

Cell Biology

Cell and Molecular Biology

Physiology

DOI

10.1016/j.ejcb.2020.151127

PubMed

33162173

More information

Latest update

12/18/2020