Novel clearance of muscle proteins by muscle cells
Artikel i vetenskaplig tidskrift, 2020

Blood levels of cardiac troponins (cTn) and myoglobin are analysed when myocardial infarction (MI) is suspected. Here we describe a novel clearance mechanism for muscle proteins by muscle cells. The complete plasma clearance profile of cTn and myoglobin was followed in rats after intravenous or intermuscular injections and analysed by PET and fluorescence microscopy of muscle biopsies and muscle cells. Compared with intravenous injections, only 5 % of cTnT, 0.6 % of cTnI and 8 % of myoglobin were recovered in the circulation following intramuscular injection. In contrast, 47 % of the renal filtration marker FITC-sinistrin and 81 % of cTn fragments from MI-patients were recovered after intramuscular injection. In addition, PET and biopsy analysis revealed that cTn was taken up by the quadriceps muscle and both cTn and myoglobin were endocytosed by cultured muscle cells. This local clearance mechanism could possibly be the dominant clearance mechanism for cTn, myoglobin and other muscle damage biomarkers released by muscle cells.

Muscle

Myoglobin

Clearance

Troponin

Endocytosis

Författare

Aida Muslimovic

Sahlgrenska universitetssjukhuset

Vincent Fridén

Sahlgrenska universitetssjukhuset

Karin Starnberg

Sahlgrenska universitetssjukhuset

Olav Tenstad

Universitetet i Bergen

Heidi Espedal

Universitetet i Bergen

Kristina Vukusic

Sahlgrenska universitetssjukhuset

Susanne Nyström

Sahlgrenska universitetssjukhuset

Emelie Vilhelmsson Wesén

Chalmers, Biologi och bioteknik, Kemisk biologi

Elin Esbjörner Winters

Chalmers, Biologi och bioteknik, Kemisk biologi

Kristoffer Granholm

Sahlgrenska universitetssjukhuset

Bertil Lindahl

Akademiska Sjukhuset

Ola Hammarsten

Sahlgrenska universitetssjukhuset

European Journal of Cell Biology

0171-9335 (ISSN)

Vol. 99 8 151127

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Fysiologi

DOI

10.1016/j.ejcb.2020.151127

PubMed

33162173

Mer information

Senast uppdaterat

2020-12-18