High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection
Journal article, 2021
Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals.
femtosecond
macromolecular crystallography.
time-over-threshold
multilayer monochromator
Author
Maja Jensen
University of Gothenburg
Viktor Ahlberg Gagnér
University of Gothenburg
Juan Cabello Sánchez
Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory
Åsa U. J. Bengtsson
Lund University
J.Carl Ekström
MAX IV Laboratory
Tinna Björg Úlfarsdóttir
University of Gothenburg
Maria-Jose Garcia-Bonete
University of Gothenburg
Andrius Jurgilaitis
MAX IV Laboratory
David Kroon
MAX IV Laboratory
Van-Thai Pham
Vietnam Academy of Science and Technology
MAX IV Laboratory
Stefano Checcia
MAX IV Laboratory
Hélène Coudert-Alteirac
MAX IV Laboratory
Siawosch Schewa
Technical University of Applied Sciences Lübeck
Manfred Rössle
Technical University of Applied Sciences Lübeck
Helena Rodilla
Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory
Jan Stake
Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory
Vitali Zhaunerchyk
University of Gothenburg
Jörgen Larsson
Lund University
Gergely Katona
University of Gothenburg
Journal of Synchrotron Radiation
0909-0495 (ISSN) 1600-5775 (eISSN)
Vol. 28 64-70Subject Categories
Accelerator Physics and Instrumentation
Atom and Molecular Physics and Optics
Structural Biology
Infrastructure
Kollberg Laboratory
Roots
Basic sciences
DOI
10.1107/S1600577520014599
PubMed
33399553
Related datasets
X-ray diffraction images of bovine trypsin crystals recorded at the FemtoMAX beamline of Max IV synchrotron facility [dataset]
DOI: 10.5281/zenodo.4290177