High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection
Journal article, 2021

Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals.

macromolecular crystallography.

femtosecond

time-over-threshold

multilayer monochromator

Author

Maja Jensen

University of Gothenburg

Viktor Ahlberg Gagnér

University of Gothenburg

Juan Cabello Sánchez

Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory

Åsa U. J. Bengtsson

Lund University

J.Carl Ekström

MAX IV Laboratory

Tinna Björg Úlfarsdóttir

University of Gothenburg

Maria-Jose Garcia-Bonete

University of Gothenburg

Andrius Jurgilaitis

MAX IV Laboratory

David Kroon

MAX IV Laboratory

Van-Thai Pham

MAX IV Laboratory

Vietnam Academy of Science and Technology

Stefano Checcia

MAX IV Laboratory

Hélène Coudert-Alteirac

MAX IV Laboratory

Siawosch Schewa

Technical University of Applied Sciences Lübeck

Manfred Rössle

Technical University of Applied Sciences Lübeck

Helena Rodilla

Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory

Jan Stake

Chalmers, Microtechnology and Nanoscience (MC2), Terahertz and Millimetre Wave Laboratory

Vitali Zhaunerchyk

University of Gothenburg

Jörgen Larsson

Lund University

Gergely Katona

University of Gothenburg

Journal of Synchrotron Radiation

0909-0495 (ISSN) 1600-5775 (eISSN)

Vol. 28 64-70

Subject Categories

Accelerator Physics and Instrumentation

Atom and Molecular Physics and Optics

Structural Biology

DOI

10.1107/S1600577520014599

PubMed

33399553

More information

Latest update

3/18/2021