High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection
Artikel i vetenskaplig tidskrift, 2021

Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals.

macromolecular crystallography.

femtosecond

time-over-threshold

multilayer monochromator

Författare

Maja Jensen

Göteborgs universitet

Viktor Ahlberg Gagnér

Göteborgs universitet

Juan Cabello Sánchez

Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik

Åsa U. J. Bengtsson

Lunds universitet

J.Carl Ekström

Max IV-laboratoriet

Tinna Björg Úlfarsdóttir

Göteborgs universitet

Maria-Jose Garcia-Bonete

Göteborgs universitet

Andrius Jurgilaitis

Max IV-laboratoriet

David Kroon

Max IV-laboratoriet

Van-Thai Pham

Max IV-laboratoriet

Vietnam Academy of Science and Technology

Stefano Checcia

Max IV-laboratoriet

Hélène Coudert-Alteirac

Max IV-laboratoriet

Siawosch Schewa

Technische Hochschule Lübeck

Manfred Rössle

Technische Hochschule Lübeck

Helena Rodilla

Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik

Jan Stake

Chalmers, Mikroteknologi och nanovetenskap (MC2), Terahertz- och millimetervågsteknik

Vitali Zhaunerchyk

Göteborgs universitet

Jörgen Larsson

Lunds universitet

Gergely Katona

Göteborgs universitet

Journal of Synchrotron Radiation

0909-0495 (ISSN) 1600-5775 (eISSN)

Vol. 28 64-70

Ämneskategorier

Acceleratorfysik och instrumentering

Atom- och molekylfysik och optik

Strukturbiologi

DOI

10.1107/S1600577520014599

PubMed

33399553

Mer information

Senast uppdaterat

2021-03-18