Convolutional neural networks for segmentation of FIB-SEM nanotomography data from porous polymer films for controlled drug release
Journal article, 2021

Phase-separated polymer films are commonly used as coatings around pharmaceutical oral dosage forms (tablets or pellets) to facilitate controlled drug release. A typical choice is to use ethyl cellulose and hydroxypropyl cellulose (EC/HPC) polymer blends. When an EC/HPC film is in contact with water, the leaching out of the water-soluble HPC phase produces an EC film with a porous network through which the drug is transported. The drug release can be tailored by controlling the structure of this porous network. Imaging and characterization of such EC porous films facilitates understanding of how to control and tailor film formation and ultimately drug release. Combined focused ion beam and scanning electron microscope (FIB-SEM) tomography is a well-established technique for high-resolution imaging, and suitable for this application. However, for segmenting image data, in this case to correctly identify the porous network, FIB-SEM is a challenging technique to work with. In this work, we implement convolutional neural networks for segmentation of FIB-SEM image data. The data are acquired from three EC porous films where the HPC phases have been leached out. The three data sets have varying porosities in a range of interest for controlled drug release applications. We demonstrate very good agreement with manual segmentations. In particular, we demonstrate an improvement in comparison to previous work on the same data sets that utilized a random forest classifier trained on Gaussian scale-space features. Finally, we facilitate further development of FIB-SEM segmentation methods by making the data and software used open access.

image analysis

porous materials

polymer films

deep learning

convolutional neural networks

machine learning

focused ion beam scanning electron microscopy

microstructure

semantic segmentation

controlled drug release

Author

Fredrik Skärberg

RISE Research Institutes of Sweden

Cecilia Fager

Chalmers, Physics, Nano and Biophysics

Royal Institute of Technology (KTH)

Francisco Mendoza-Lara

AstraZeneca AB

Mats Josefson

AstraZeneca AB

Eva Olsson

Chalmers, Physics, Nano and Biophysics

Niklas Lorén

RISE Research Institutes of Sweden

Chalmers, Physics, Nano and Biophysics

Magnus Röding

RISE Research Institutes of Sweden

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

Journal of Microscopy

0022-2720 (ISSN) 1365-2818 (eISSN)

Vol. 283 1 51-63

Subject Categories

Polymer Chemistry

Polymer Technologies

Other Chemistry Topics

DOI

10.1111/jmi.13007

PubMed

33797085

More information

Latest update

6/28/2021