A Novel Graphene Quantum Dot-Based mRNA Delivery Platform
Journal article, 2021
During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
functionalization
mRNA
hepatocarcinoma
graphene quantum dots
drug delivery
Author
Ya Liu
Fudan University
Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems
Changhong Zhao
Xinxiang Medical University
Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems
Alan Sabirsh
AstraZeneca R&D
Lilei Ye
SHT Smart High-Tech
Xiaoqiu Wu
AstraZeneca R&D
Hongbin Lu
Fudan University
Johan Liu
Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems
Shanghai University
ChemistryOpen
2191-1363 (eISSN)
Vol. 10 7 666-671Subject Categories
Cell Biology
Cell and Molecular Biology
Pharmacology and Toxicology
Areas of Advance
Production
DOI
10.1002/open.202000200
PubMed
33829677