A Novel Graphene Quantum Dot-Based mRNA Delivery Platform
Artikel i vetenskaplig tidskrift, 2021
During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
functionalization
mRNA
hepatocarcinoma
graphene quantum dots
drug delivery
Författare
Ya Liu
Fudan University
Chalmers, Mikroteknologi och nanovetenskap, Elektronikmaterial
Changhong Zhao
Xinxiang Medical University
Chalmers, Mikroteknologi och nanovetenskap, Elektronikmaterial
Alan Sabirsh
AstraZeneca R&D
Lilei Ye
SHT Smart High-Tech
Xiaoqiu Wu
AstraZeneca R&D
Hongbin Lu
Fudan University
Johan Liu
Chalmers, Mikroteknologi och nanovetenskap, Elektronikmaterial
Shanghai University
ChemistryOpen
2191-1363 (eISSN)
Vol. 10 7 666-671Ämneskategorier
Cellbiologi
Cell- och molekylärbiologi
Farmakologi och toxikologi
Styrkeområden
Produktion
DOI
10.1002/open.202000200
PubMed
33829677