Analytical performance of a standardized kit for mass spectrometry-based measurements of human glycosaminoglycans
Journal article, 2021

Glycosaminoglycans (GAGs) are long linear sulfated polysaccharides implicated in processes linked to disease development such as mucopolysaccharidosis, respiratory failure, cancer, and viral infections, thereby serving as potential biomarkers. A successful clinical translation of GAGs as biomarkers depends on the availability of standardized GAG measurements. However, owing to the analytical complexity associated with the quantification of GAG concentration and structural composition, a standardized method to simultaneously measure multiple GAGs is missing. In this study, we sought to characterize the analytical performance of a ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-MS/MS)-based kit for the quantification of 17 free GAG disaccharides. The kit showed acceptable linearity, selectivity and specificity, accuracy and precision, and analyte stability in the absolute quantification of 15 disaccharides. In native human samples, here using urine as a reference matrix, the analytical performance of the kit was acceptable for the quantification of CS disaccharides. Intra- and inter-laboratory tests performed in an external laboratory demonstrated robust reproducibility of GAG measurements showing that the kit was acceptably standardized. In conclusion, these results indicated that the UHPLC-MS/MS kit was standardized for the simultaneous measurement of free GAG disaccharides allowing for comparability of measurements and enabling translational research.

UHPLC-MSMS

Oligosaccharides

Glycosaminoglycans

Analytical validation

Author

Davide Tamburro

Elypta AB

Sinisa Bratulic

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Souad Abou Shameh

Elypta AB

Nikulkumar Soni

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology, CMSI

Andrea Bacconi

Elypta AB

F. Maccari

University of Modena and Reggio Emilia

F. Galeotti

University of Modena and Reggio Emilia

Karin Mattsson

Elypta AB

N. Volpi

University of Modena and Reggio Emilia

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

BioInnovation Institute

Francesco Gatto

Elypta AB

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

1570-0232 (ISSN)

Vol. 1177 122761

Subject Categories

Medical Laboratory and Measurements Technologies

Analytical Chemistry

Biomedical Laboratory Science/Technology

DOI

10.1016/j.jchromb.2021.122761

PubMed

34052753

More information

Latest update

6/30/2021