Microbial production of chemicals driven by CRISPR-Cas systems
Review article, 2022

Microorganisms have provided an attractive route for biosynthesis of various chemicals from renewable resources. CRISPR-Cas systems have served as powerful mechanisms for generating cell factories with desirable properties by manipulating nucleic acids quickly and efficiently. The CRISPR-Cas system provides a toolbox with excellent opportunities for identifying better biocatalysts, multiplexed fine-tuning of metabolic flux, efficient utilization of low-cost substrates, and improvement of metabolic robustness. The overall goal of this review highlights recent advances in the development of microbial cell factories for chemical production using various CRISPR-Cas systems. The perspectives for further development or applications of CRISPR-Cas systems for strain improvement are also discussed.


Shuobo Shi

Beijing University of Chemical Technology

Nailing Qi

Beijing University of Chemical Technology

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Beijing University of Chemical Technology

BioInnovation Institute

Current Opinion in Biotechnology

0958-1669 (ISSN) 1879-0429 (eISSN)

Vol. 73 34-42

Subject Categories

Biochemistry and Molecular Biology


Bioinformatics and Systems Biology





More information

Latest update

8/9/2021 7