Aborting meiosis allows recombination in sterile diploid yeast hybrids
Journal article, 2021

Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.

Author

Simone Mozzachiodi

University of Côte d'Azur

Lorenzo Tattini

University of Côte d'Azur

Agnès Llored

University of Côte d'Azur

Agurtzane Irizar

University of Côte d'Azur

Neža Škofljanc

University of Côte d'Azur

Melania D’Angiolo

University of Côte d'Azur

Matteo De Chiara

University of Côte d'Azur

Benjamin P. Barré

University of Côte d'Azur

Jia Xing Yue

Sun Yat-sen University Cancer Center

Angela Lutazi

University of Côte d'Azur

Sophie Loeillet

Institut Curie

Raphaelle Laureau

Columbia University

Souhir Marsit

Institut Curie

Simon Stenberg

Student at Chalmers

Benoit Albaud

Institut Curie

Karl Persson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Jean-Luc Legras

University of Montpellier

Sylvie Dequin

University of Montpellier

Jonas Warringer

University of Gothenburg

Alain Nicolas

University of Côte d'Azur

Gianni Liti

University of Côte d'Azur

Nature Communications

2041-1723 (ISSN)

Vol. 12 6564

Subject Categories

Evolutionary Biology

Cell Biology

Microbiology

Genetics

DOI

10.1038/s41467-021-26883-8

More information

Latest update

1/18/2022