Aborting meiosis allows recombination in sterile diploid yeast hybrids
Artikel i vetenskaplig tidskrift, 2021

Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.

Författare

Simone Mozzachiodi

Université Côte d'Azur

Lorenzo Tattini

Université Côte d'Azur

Agnès Llored

Université Côte d'Azur

Agurtzane Irizar

Université Côte d'Azur

Neža Škofljanc

Université Côte d'Azur

Melania D’Angiolo

Université Côte d'Azur

Matteo De Chiara

Université Côte d'Azur

Benjamin P. Barré

Université Côte d'Azur

Jia Xing Yue

Sun Yat-Sen University

Angela Lutazi

Université Côte d'Azur

Sophie Loeillet

Institut Curie

Raphaelle Laureau

Columbia University

Souhir Marsit

Institut Curie

Simon Stenberg

Student vid Chalmers

Benoit Albaud

Institut Curie

Karl Persson

Göteborgs universitet

Jean-Luc Legras

Université de Montpellier

Sylvie Dequin

Université de Montpellier

Jonas Warringer

Göteborgs universitet

Alain Nicolas

Université Côte d'Azur

Gianni Liti

Université Côte d'Azur

Nature Communications

2041-1723 (ISSN) 20411723 (eISSN)

Vol. 12 6564

Ämneskategorier

Evolutionsbiologi

Cellbiologi

Mikrobiologi

Genetik

DOI

10.1038/s41467-021-26883-8

Mer information

Senast uppdaterat

2022-07-25