Aborting meiosis allows recombination in sterile diploid yeast hybrids
Artikel i vetenskaplig tidskrift, 2021
Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.
Författare
Simone Mozzachiodi
Université Côte d'Azur
Lorenzo Tattini
Université Côte d'Azur
Agnès Llored
Université Côte d'Azur
Agurtzane Irizar
Université Côte d'Azur
Neža Škofljanc
Université Côte d'Azur
Melania D’Angiolo
Université Côte d'Azur
Matteo De Chiara
Université Côte d'Azur
Benjamin P. Barré
Université Côte d'Azur
Jia Xing Yue
Sun Yat-Sen University
Angela Lutazi
Université Côte d'Azur
Sophie Loeillet
Université Pierre et Marie Curie (UPMC)
Raphaelle Laureau
Columbia University
Souhir Marsit
Université Pierre et Marie Curie (UPMC)
Simon Stenberg
Student vid Chalmers
Benoit Albaud
Université Pierre et Marie Curie (UPMC)
Karl Persson
Göteborgs universitet
Jean-Luc Legras
Université de Montpellier
Sylvie Dequin
Université de Montpellier
Jonas Warringer
Göteborgs universitet
Alain Nicolas
Université Côte d'Azur
Gianni Liti
Université Côte d'Azur
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 12 6564Ämneskategorier (SSIF 2011)
Evolutionsbiologi
Cellbiologi
Mikrobiologi
Genetik
DOI
10.1038/s41467-021-26883-8